Histone 3 抗体 (pSer28)
Quick Overview for Histone 3 抗体 (pSer28) (ABIN6972062)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer28
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产品特性
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. Ser10 phosphorylation and Ser28 phosphorylation in the tail of H3 have very similar kinetics. Both phosphorylations occur early in mitosis when chromosomes begin to condense and during premature chromosome condensation induced in S-phase cells. These phosphorylated serines are excellent mitotic markers. In contrast to Ser10 phosphorylation, Ser28 phosphorylation has never been observed in interphase. Histone H3S28ph antibody (mAb) (Clone 5F9A9) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
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纯化方法
- Protein A Chromatography
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免疫原
- This antibody was raised against a peptide corresponding to phospho-serine 28 of human Histone H3.
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亚型
- IgG2b
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anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (3meLys4) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone H3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (C-Term) antibodyH3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K9me2) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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缓冲液
- Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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分子量
- 17 kDa
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NCBI登录号
- NP_003522
抗原
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